Tuesday, February 4, 2025

In memory of our friend David Johnson

 



David Alfred Johnson Ph.D

2 September 1931 -  4 February 2025


All of us of project GRFT lost a very good friend today



David was one of the project GRFT team from the start.  He specialized in hardware engineering and development. He took charge of particle bombardment technology being used by project GRFT.  He had an interest in this and stated that he thought there were many applications which this technology would be used for in the future and this was only the beginning.  He was inspired by the DIY gene gun that had been built at BioCurious by Jay Hanson.  Based on this design, he built two DIY gene guns for project GRFT. He designed 3D printed nozzles that were optimized for particle spread.  David made many tests with the Gene guns to get the gas pressure adjusted for best cell penetration.  He learned the basics of plant molecular biology in an amazingly short time and helped to get the contamination issues that we were having under control.  

Project GRFT is where it is today, with the micropropagation pipeline in place and gene gun working for transformations, largely because of the knowledge, experience and skills he brought to us.   I personally enjoyed every minute in the lab with David, and I am sure that all of us who had the pleasure of knowing him can say the same.  He was at most of our meetings over the years.  Sometimes he would have trouble with the audio settings on his device, but he would get that working and then have insights to share with us.  

David was thoughtful with the analytical skill set of a great scientist.  He worked very hard at CCL and got a lot accomplished. I was always hugely impressed by his abilities to work with a number of different technologies, and to see clearly how they all interact.  Besides being a top scientist and inventor, David had may interests and abilities, for example he is an artist and a poet.  David had heart problems.  He said he wasn't in much pain and that he got tired easily.  Little over a week ago during one on the walks that he took for fresh air and exercise, his heart gave out and he collapsed which caused multiple severe injuries.  He was taken to Highland Hospital.  I went to visit him, and he was having trouble breathing. He couldn't swallow or talk.  His wife Leticia was staying with him at the hospital and caring for him tenderly.  I came back the next day, the doctors had changed his meds.  He was breathing without a struggle and looked peaceful.  

      



Ph.D. Physics (1972)

University of Hawaii. Honolulu, HI.

Dr. Johnson worked as a physist at UC Berkeley Lawrence Lab and the Stanford Linear Accelerator Center.

He did much pioneering work with high energy physics.

Dr. Johnson was the founder, director, and president of TiNi Alloy Company where he managed a research team engaged in development of products using shape-memory alloys.

He owns multiple patents and has published papers.

One of his inventions has travelled to the planet Mars

                               David and Hector at CCL

Sunday, September 22, 2024

WHO Geneva: awareness of a viral problem

“The next pandemic will not wait for us, whether from a flu virus like H5N1, another coronavirus, or another family of viruses we don’t yet know about,” Dr Tedros told the meeting. “But all the ingredients are in place to meet the objective of countries to negotiate a generational pandemic agreement. The world needs hope that it is still possible for countries to find common solutions to common problems. You can provide that hope.”


https://www.who.int/news/item/20-09-2024-governments-progress-on-negotiations-for-a-pandemic-agreement-to-boost-global-preparedness-for-future-emergencies

project GRFT continues our efforts to prepare for future emergencies. We hope that these efforts will help to realize our common goals of keeping our future free of pandemics. 





Saturday, August 17, 2024

Mpox

 There's currently a Mpox virus outbreak.  Mpox, formerly known as monkeypox, is a virus that transmits from animals to animals and also from animals to humans (zoonotic transmission) and from humans to humans.  It has been observed in humans since 1970.

Many of the human to human transmissions come from exchange of bodily fluids through sexual contact, however there are also other pathways for infection to occur.

This virus is mutating and is often lethal so it's a real cause for concern.

A number of antivirals are being tested against Mpox

Could GRFT stop the progress of this epidemic?

We don't have an answer to that question as we haven't seen any research being done on GRFT vs Mpox yet.

If anyone has information regarding research being done using the anti-viral properties of GRFT to stop Mpox, please pass that on to us.

It seems probable that as Mpox is an enveloped virus, GRFT should be able to stop transmission into cells but we are waiting for the research to confirm this hypothesis.

Sunday, July 21, 2024

Nipah Virus: the next epidemic?

 A 14 year old boy has been reported to have died from a Nipah Virus infection.  This happened in Kerala state in India.  This location is an area of concern and there have been multiple deaths from Nipah virus since 2018.  

There is a high potential for an outbreak originating in the Kerala area to become an epidemic. 

Can GRFT (griffithsin) play a role in preventing the spread of this lethal virus?  

A 2020 paper published in The Journal of Infectous Diseases details in vitro research and from the results of this study concludes "Our findings, taken together with the favorable pharmacokinetic and safety profiles of GRFT, make Q-GRFT an excellent candidate for further preclinical evaluation against NiV infection."

sources:

 https://www.bbc.com/news/articles/cj50d7e9vp6o

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199786/

Sunday, July 7, 2024

Year of the elusive plasmid

 For the last year, starting in July 2023, we at CCL have been attempting to assemble and verify a plasmid to use in the transformation of rice plants.  Two scientists from Johns Hopkins University visited us last July.  They brought the insert containing the gene to express GRFT.  Having no previous experience with plasmid assembly, this task involves a learning curve for me.  There have been multiple fails which are among other things, teachers.   We try not to repeat the same mistakes and like to think we are making progress.  

The situation with CCL seems more hopeful than it was during the last post I made here.  CAST is stepping in to see if they can purchase the Omni Commons.  In that case our lab is secured from having to wander about looking for a new home.   

It's an interesting summer that hasn't left much time for posting on this blog.  We're still alive and kickin'.