powerful antiviral lectin meets computer game

Soooo creative....

http://www.nature.com/ncomms/2016/160916/ncomms12549/full/ncomms12549.html


http://www.nature.com/ncomms/2016/160916/ncomms12549/extref/ncomms12549-s2.mov

Excerpt from protocol/labnotes

Excerpt from the protocol:

Nenufar – antiviral research project 01 – Expression and purification of peptide LBOUNCER from the synthetic  gene VRBNCR-01 inserted into an e-coli plasmid vector, transformed and induced to grow on bacteria.  Explore how to do this in a fast and consistent way with the highest purity.

Experiment 01A:

DNA synthesis of the VRBNCR-01 gene which is inserted into a pET15 expression plasmid with a His 6 tag on the N-Terminus followed by a thrombin cleavage site.  The gene will be cloned into pET15b via NdeI and BamHI. We will use heat transform into competent cells, then plate out on Amp-agar to grow colonies.  Induce for expression.

Then on to Experiment 01B: purification using chromatography and SDS page

I contacted a lab in the San Francisco Bay Area where we can work.  All in all, we are going to do a lot of improvising so we can stretch the budget and work for at least 6 months on the experiment. Everyone is volunteering their time. We have wonderful mentors and we are learning by doing, the biohacker way.  It is always a steep learning curve as anyone who has worked in molecular biology knows.  The journey of discovery makes it worthwhile.  Thanks to all of you who are making this possible.

bananas

https://www.youtube.com/watch?v=DFrLcNk0GWU