Friday, May 27, 2016

Basic skills in the lab - the fine art of pipetting

Those of us doing molecular biology wetwork in the lab are as much connected to pipettes as most of the general public are to mobile phones.

As any precision instrument, pipettors and tips must be handled gently and with care, of course paying attention to sterility.  Experienced biohackers who should know better often use improper techniques that cause one to cringe.  

There are basic pipette skills which are essential to learn, but it doesn't end there, it is really an art.

So in this post I'm putting in a few links to learning the fine art of pipetting, from the basics to more advanced techniques.  Please add any links or pipetting stories you may have to share.  

First, there is an advanced level webinar on May 31, 2016 

Protect your Cells with Proper Pipetting - How liquid handling influences your cell culture work


also an archived webinar:

For the basics, there are a number of good videos





We get a lot of used pipettors, What needs to be done to make sure that they are accurate?  Calibration.


serial dilution - multi-channel





Happy pipetting, brothers and sisters!






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