Friday, November 11, 2022

Project GRFT gene gun

 






project GRFT has set up our first gene gun.  It was designed and built by physicist and inventor David Johnson PhD.  In the video he fires the first shot from the gene gun in the new lab environment.  DNA plasmid coated Au microparticles are shot into a rice callus which will then be incubated at 25C.  Compressed Helium gas provides the force to propel the particles into the plant cells.  

Monday, November 7, 2022

project GRFT does particle bombardment

 Project GRFT, after setting up our gene gun is shooting DNA coated microparticles into rice calluses.

Two of our volunteers, Kat and Patrick, in the hood and washing the Au microparticles with ethanol.



They are using molecular biology grade ethanol and we do 3 washes


after adding the ethanol -
time to vortex!