work in progress

Thanh's gene gun rapid fire magazine design for our hardware working group.

 

Please help support the project:

(new link)

https://charity.gofundme.com/o/en/campaign/help-us-build-a-gene-gun

protein expression virtual event 10May2017

Sign up and attend this interactive event on Wednesday.

https://www.labroots.com/ms/virtual-event/gibco-expressionworld-advancing-world-protein-expression



Log in anytime May 10, 2017 after 8:00 am regardless of your time zone

Attendees will receive first-hand knowledge of the newest technologies for high-titer recombinant protein production as well as protein expression system design, workflows, purification, and characterization methods. 

Through the looking glass - 2017 update

Here’s some of what I saw this year at BiOS and represents only a small fraction of what was there.  I’ll put up a few more links soon.  It is fascinating to see all these amazing developments in biophotonics, biomedical optics, and imaging being made at such a rapid pace.  Thanks to all who graciously let me point my little camera at them.  Please visit their linked websites to learn more about what is outlined here.

In the order that they appear in the video we present:

Dipl. - Phys. Uwe Ortmann of  PicoQuant returned this year from Berlin with the latest Fluorescence Lifetime Imaging (FLIM) development to capture motion in cells at 10 frames per second (FPS). 

http://www.picoquant.com/

Aileen Chen of Vista Optronics Inc. came to San Francisco from China and demonstrated a tiny endoscope (1.5mm outside diameter) - not much larger than an acupuncture needle. 

www.VistaOptronics.com

Justin Harris Ph.D. of  NanoHybrids Advanced Imaging Solutions came from Austin, Texas and explained how gold and light are used for non-invasive cancer diagnosis and treatment.

www.NanoHybrids.net

Markus Geiser Dr. sc. ETH of  IRsweep came from Zurich Switzerland and demonstrated a new spectrometer with  bandwidth of up to 150 cm^-1 in the mid-IR range of 5 µm (2000 cm^-1) to 10 µm (1000cm^-1).

www.irsweep.com

Ben Alexander of Caliber Imaging and Diagnostics from Lakeway, Texas demonstrated a new technology for quickly scanning and stitching together confocal microscope images of biological samples up to 80 x 120 mm.

www.caliberid.com

CRISPR-cas9 gene editing vs metastatic non-small-cell lung cancer

Yes, in humans.  

http://www.nature.com/news/crispr-gene-editing-tested-in-a-person-for-the-first-time-1.20988

If successful, this could be a major development in a model of therapy for curing many diseases.

CRISPR cas-9 (More than 2 million people watched this)

CRISPR: An enzyme taken from Leptotrichia shahii can bind to and cut ss messenger RNA.

A new version of the gene-editing tool CRISPR can target and cut RNA

also see:

http://www.the-scientist.com/?articles.view/articleNo/46234/title/RNA-Targeting-CRISPR/

and

http://www.the-scientist.com/?articles.view/articleNo/45354/title/Dial-It-Up--Dial-It-Down/

Lab management

I found a service that looks useful for anyone running a lab, is anyone using Quartzy? 

https://www.quartzy.com/

planning stages underway

It's a rainy day, much welcome in this drought-stricken state (California).   I hope everyone had a nice holiday. Here we are writing and revising our design and protocol for the premier experiment. We'll post more about this as we progress.  We will be making a propaganda video to raise the financing we need to get into the lab with all our materials and equipment ready to go.  Since we may or may not be successful at first, we'll work on raising enough for several experiments, fine tuning each time until we get the desired result.  Thanks for reading.  

BLOG INAUGURATION

BLOG INAUGURATION

This blog could also be titled "adventures in biology" because it is going to chronicle our journeys into the life of cells and molecules.  The San Francisco Bay Area is a brilliant point of light in the global science network (You knew that, didn't you?). I'd like to thank the citizen scientists of the Bay Area biohacker community for your kind help and support.  I'm not mentioning any names in this post but you know who you are.  Nenufar loves you!
Nenufar is preparing to conduct our first experiment to produce a peptide,  Hey, why don't we give it a name?

NML-0001

Our interest in lectins with antiviral properties led to research that indicates that this peptide may have medical applications.  We will say more about this in future posts.

We will welcome any interested parties interested in science to join with us on this adventure,  please leave your comments and questions as the experiment progresses.